Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Capsular polysaccharide typing of domestic mastitis-causing Staphylococcus aureus strains and its potential exploration of bovine mastitis vaccine developmen | J. Vet. Sci. 2000 1 1 53-60 JOURNAL OF Veterinary Science Capsular polysaccharide typing of domestic mastitis-causing Staphylococcus aureus strains and its potential exploration of bovine mastitis vaccine developmen. I. capsular polysaccharide typing isolation and purification of the strains Hong-Ryul Han Son-Il Pak Seung-Won Kang Woo-Seog Jong1 Cheol-Jong Youn2 Department of Internal Medicine College of Veterinary Medicine Seoul National University Seoul 151-742 Korea 1 National Veterinary Research and Quarantine Service Anyang 430-016 Korea Department of Pathology College of Medicine Seoul National University Seoul 110-744 Korea One hundred seven isolates of Staphylococcus aureus from bovine mastitis were investigated for colony morphology in serum-soft agar SSA autoagglutination in salt and capsular serotype. Capsular polysaccharide CP was purified and quantified from the extracts of clinical isolates. Overall 89 isolates were diffuse in the SSA without any difference in the proportion of diffuse colony between type 5 and type 8 strains. Some strains exhibited compact colonies in the SSA and expressed CP as determined by an enzyme-linked immunosorbent assay indicating that compact morphology does not exclude encapsulation. The majority of the strains 11 12 showed autoagglutination in the salt aggregation test. The serotype 336 accounted for of the isolates followed by serotype 5 and serotype 8 . Particularly twenty-six isolates reacted with two serotypes 7 for type 8 336 and 19 for type 5 336. Five isolates were nontypeable with monoclonal antibodies specific for CP serotype 5 8 or 336. The CP concentration in culture supernatants varied with the serotypes and the total amount of CP produced by cells grown in a liquid medium was much less than that produced by cells grown on a solid medium. The Western blotting indicated that the CP bands of S. aureus serotype 5 and 8 were ranged in the molecular mass of 58-84 kilodalton kDa with .
