Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Study on mechanism of multistep hepatotumorigenesis in rat: development of hepatotumorigenesis | J. Vet. Sci. 2001 2 1 53-58 JOURNAL OF Veterinary Science Study on mechanism of multistep hepatotumorigenesis in rat development of hepatotumorigenesis Woo-song Ha1 Chi-kyeong Kim Seung-hee Song and Chung-boo Kang Department of Internal Medicine College of Veterinary Medicine and Institute of Animal Medicine Gyeongsang National University Chinju 660-701 Korea Department of Surgery College of Medicine GyeongsangNational University Chinju 660-701 Korea With the aim of establishing bio-indices for the development of multistep hepatotumorigenesis rats were fed water containing diethylnitrosamine DEN ad libitum for 13 weeks. This treatment with DEN only made it possible to induce hepatic tumors in 100 . After the DEN administration several clinical symptoms were observed including minor behavioral changes brittleness of hair and a decrease in water and food intake. The concentration of total serum protein and albumin in all treated groups was significantly lower than in non-treated controls P . Increase of specific enzyme AST ALT and GGT activity P variable tumor size and hepatomegaly of the liver was observed in all rats treated with DEN for 10 weeks. Both hepatocellular carcinoma and cholangiocarcinoma were found in the same livers at the same time and were prominently developed after 12 weeks. In case of carcinoma some of the livers showed more or less advanced states over the 12-15 weeks period. In the present study hepatocellular carcinoma was developed by treating DEN in only the drinking water without any other carcinogens or without partial hepatectomy. These results indicate that DEN is a new carcinogen that acts directly on it the liver moreover it might be very useful for investigating hepatotumorigenesis. Key words Diethylnitrosamine tumorigenesis hepatocellular carcinoma enzyme activity rat. Introduction Hepatocellular carcinoma can be induced in the livers of laboratory animals by a variety of chemicals 1 3 10 13 14 16 17 20 23 . It has .
