Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Tissue distribution of bovine viral diarrhea virus antigens in persistently infected cattle Taekyun Shin* and Helen Acland1 | J. Vet. Sci. 2001 2 2 81-84 OURNAL OF Veterinary Science Tissue distribution of bovine viral diarrhea virus antigens in persistently infected cattle Taekyun Shin and Helen Acland1 Department of Veterinary Medicine Institute of Animal Science College of Agriculture Cheju National University Jeju 690-756 Korea 1 Pennsylvania Veterinary Laboratory Harrisburg PA 17110 USA The tissue distribution and cellular localization of viral antigens in three cattle with persistent bovine viral diarrhea virus BVDV infection was studied. In three cases necropsy findings of oral ulcers abmasal ulcers and necrosis of Peyer s patches were suspected have been caused by BVDV infection. Non-cytopathic BVDV was isolated from a tissue pool of liver kidneys and spleen. Immunohistochemical detection of BVDV showed that BVDV antigens were detected in both epithelial and non-epithelial cells in all examined organs including the gastrointestinal tract liver pancreas lung lymphatic organs spleen lymph nodes adrenal gland ovary uterus and the mammary gland. These findings support the hypothesis that animals with persistent BVDV infection spread BVDV through all routes and that infertility in BVDV infection is associated with the infection of BVDV in the ovaries and uteri. Key words Bovine diarrhea virus cattle diagnosis Introduction Bovine viral diarrhea virus BVDV is a positive-sense single-strand RNA virus. BVDV is one of the most important viral pathogens of cattle and its control and prevention are of worldwide concern. Moreover BVDV infection has been associated with enteric disease mucosal disease 2 diabetes 12 and reproductive failure 1 8 9 . The reproductive effects of BVDV infection include early embryo loss abortion and congenital defects 9 . Several studies have shown that a variety of organs including the lymph nodes spleen and liver are preferred sites of viral replication in fetuses and adult cattle 3 4 7 10 . Recently ovaries have been shown to be one of the possible sites of BVDV
