Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Studies of cocktail therapy with multiple cytokines for neoplasia or infectious disease of the dog | J. Vet. Sci. 2001 2 2 115-120 OURNAL OF Veterinary Science Studies of cocktail therapy with multiple cytokines for neoplasia or infectious disease of the dog I. cDNA cloning of canine IL-3 and IL-6 Il-seob Shin Hye-ryon Kim Myung-jin Nam1 and Hwa-young Youn Department of Veterinary Internal Medicine College of Veterinary Medicine and School of Agricultural Biotechnology Seoul National University Seoul 151-742 Korea Cancer Research Division National Institute of Health Seoul 122-701 Korea This paper describes the cloning and sequence analysis of the cDNAs encoding the canine homologues of interleukin-3 IL-3 and interleukin-6 IL-6 . The coding sequences for canine IL-3 and IL-6 were obtained by using the reverse transcription polymerase chain reaction RT-PCR with RNA harvested from canine peripheral blood mononuclear cells PBMCs . Canine IL-3 cDNA includes a single open reading frame of 432 nucleotides which encodes a 143 amino acid polypeptide and has 37 and homology with the cow sheep human and rat IL-3 sequences respectively. Canine IL-6 cDNA GenBank accession number AF275796 encodes a putative 20-amino acid signal peptide followed by a 187-amino acid mature protein. The predicted amino acid sequence of canine IL-6 shares and sequence identity with those of human feline porcine sheep and rat IL-6 respectively. Key words Cytokine cDNA cloning PCR IL-3 IL-6 dog Introduction Interleukin-3 IL-3 is a glycoprotein which has a broad spectrum colony stimulating effect. IL-3 acts on primitive pluripotent stem cells and progenitor cells of every lineage except for those committed to the T-lymphoid and B-lymphoid lineages. IL-3 is also called multi-colony stimulating factor CSF mast-cell growth factor or stem cell activating factor and has many other names 15 . Thus IL-3 can stimulate the generation and differentiation of macrophages neutrophils eosinophils basophils mast cells megakaryocytes and erythroid cells 24 . Furthermore IL-3 .
