Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Angiogenesis effects of nerve growth factor (NGF) on rat corneas | J. Vet. Sci. 2001 2 2 125-130 OURNAL OF Veterinary Science Angiogenesis effects of nerve growth factor NGF on rat corneas Kangmoon Seo Jongil Choi Myungjin Park1 and Changhun Rhee1 Department of Veterinary Medicine Kangwon National University Chuncheon 200-701 Korea Dab. of Cell Biology Korea Cancer Hospital Seoul 139-706 Korea This study was performed to evaluate the effects of nerve growth factor NGF upon angiogenesis in the rat cornea to examine its possible application as an alternative angiogenic inducer and to provide basic data for further studies. Angiogenesis was induced by cornea micropocket assay as previously described. Eight of thirty two eyes of Sprague-Dawley rats were randomly assigned to one of four groups namely a non-NGF group Group 0 a ng of NGF group Group a ng of NGF group Group and a ng of NGF group Group . Pellets made of poly-2-hydroxylethylmethacrylate and sucralfate were implanted into the corneal stroma no closer than 1 mm from the limbus. After the implantation the number of new vessels vessel length and circumferential neovascularization were examined daily under the surgical microscope over a period of 7 days. The area of neovascularization was determined using a mathematical formula. Although new vessels in Group 0 and Group were first observed at day 5 those of Groups and were first noted on days 4 and 3 respectively. However the growth rates of new vessels in Groups and were higher than those of Groups 0 and with the passage of time. The number length circumferential neovascularization and areas covered by the vessels in Groups and were significantly more than in Group 0 and Group p . This study showed that NGF had a dose-dependent angiogenic effects on the rat cornea and that the minimal effective dose of NGF was ng per cornea. Also it showed that NGF would be useful in angiogenic studies as an alternative angiogenic inducer. Key words Nerve growth factor NGF .
