Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Cloning a new allele form of bovine TNF-α Jongsam Ahn | J. Vet. Sci. 2001 2 2 139-141 OURNAL OF Short communication Veterinary Science Cloning a new allele form of bovine TNF-a Jongsam Ahn Division of Bacteriology and Immunology National Veterinary Research and Quarantine Service Anyang 430-824 Korea Although little is known on the function of y8 T lymphocytes there is increasing evidence that 76 T lymphocytes are early responders and modulators of immune responses against pathogens and cytokines such as IL-2 IL-7 IL-15 and TNF-a. To study the role TNF-a on y6 T lymphocytes we cloned bovine TNF-a. Sequence analysis revealed that a new allele form of bovine TNF-a was cloned which has 3 additional nucleotide sequences as well as 3 nucleotide substitutions compared with previously reported bovine TNF-a. Further studies are needed to document the functional significance of a new allele form of TNF-a in cattle. Key words New allele bovine TNF-a T cells can be distinguished on the expression of ap or y8 forms of T cell receptor. While ap T lymphocytes are well characterized with respect to phenotype and function little is known about y8 T lymphocytes. Since y8 T lymphocytes are predominantly localized in epithelia it has been hypothesized to play in the first line of defense against infectious agents 12 13 14 . Although it is not clear the role of y8 T lymphocytes there is increasing evidence that y8 T lymphocytes are early responders and modulators of immune responses to infectious agents 1 5 6 9 11 15 19 21 . In most species examined thus far y8 T lymphocytes comprise only a small proportion 10 of T lymphocytes in peripheral blood 8 . In contrast y8 T lymphocytes comprise 30-60 of peripheral blood lymphocytes in cattle 18 20-60 in sheep 17 and 40-60 in pigs 2 . The large population of y8 T lymphocytes in ruminants and pigs is attributed to the presence of a unique subpopulation of WC1 y8 T lymphocytes that express CD3 and CD5 but not CD2 or CD6 3 4 16 20 25 . Comparative studies revealed the presence of WC1- y8 T .
