Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Immunological Responses of Dogs Experimentally Infected with Dirofilaria immitis | J. Vet. Sci. 2002 3 2 109-114 JOURNAL OF V eterinary Science Immunological Responses of Dogs Experimentally Infected with Dirofilaria immitis Kun-Ho Song Mineo Hayasaki1 Chusnul Choliq2 Kyu-Woan Cho Hong-Ryul Han3 Bung-Hyun Jeong4 Moo-Hyung Jeon5 Bae-Kun Park6 and Duck-Hwan Kim Department of Veterinary Internal Medicine Department of Veterinary Microbiology and Department of Veterinary Parasitology College of Veterinary Medicine Chungnam National University Daejeon 305-764 Korea IVeterinary Clinical Center School of Veterinary Medicine Yamaguchi University Yamaguchi 753-8515 Japan 2Veterinary Clinical Center Faculty of Veterinary Medicine Bogor Agricultural University Bogor 16151 Indonesia Department of Veterinary Internal Medicine College of Veterinary Medicine Seoul National University Seoul 151-742 Korea Department of Veterinary Internal Medicine College of Veterinary Medicine Kon-kuk University Seoul 143-701 Korea Received Feb. 3 2002 Accepted May 7 2002 ABSTRACT Three dogs were experimentally infected with Dirofilaria immitis. All dogs were euthanised at 30 36 and 37 weeks after inoculation of D. immitis for the recovery of adult worms. Three cases accounted to recovery of inoculated worms. Serum samples from dogs experimentally inoculated with D. immitis were analyzed by ELISA and immunoblotting methods. Antibody titers of dogs detected by ELISA pe aked betw een 7 and 14 weeks then decreased betw een weeks 15 to 24 follow ed by another increase during weeks 25 to 30 and persisted throughout the rem ainde of the experiment period. Analysis of adult D. immitis p rotein stained with Coom assie brilliant blue R-250 indicated sep arately more than 10 bands and the major bands were 22 40 46 56 70 72 and 89 kD a. Antigenic identification of extracts antigens of ad ults D. immitis by immunoblotting analysis revealed several bands from pooled sera of patent infection 30 w eeks after inoculation . The detected bands were 24 70 80 and 110 kDa 22 72 and 84 kDa and
