Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp Original article đề tài: Mycorrhizal associations of nursery grown Scots pine (Pinus sylvestris L.) seedlings in Poland. | Ann. For. Sci. 63 2006 715-723 INRA EDP Sciences 2006 DOI forest 2006052 715 Original article Mycorrhizal associations of nursery grown Scots pine Pinus sylvestris L. seedlings in Poland Michaỉ IWAÉSKI Maria RUDAWSKA Tomasz LESKI Institute of Dendrology Polish Academy of Sciences Kórnik Poland Received 26 October 2005 accepted 27 January 2006 Abstract - We investigated the species richness and composition of mycorrhizal fungi naturally colonizing one- and two-year-old Pinus sylvestris L. seedlings from four bare-root nurseries in Poland. We hypothesized that both edaphic factors and seedling age would affect ectomycorrhizal ECM community structure. We assessed the relative abundance of mycorrhizal morphotypes and distinguished ECM fungi present on root tips using RFLP restriction fragment length polymorphism of the ITS internal transcribed spacer of fungal rDNA. Most of the RFLP types were identified by comparing RFLP patterns with reference data obtained from regional sporocarps and isolates of ectendomycorrhizal species. Samples of unidentified RFLP types and representatives of suilloid RFLP patterns were sequenced and compared with available sequences. Overall we identified 13 fungal species with species richness varying from two to eight species among nurseries. The dominant species in each examined nursery were Wilcoxina mikolae and Thelephora terrestris. Suilloid fungi were also abundant in most of the nurseries. 5. variegatus was the most frequent suilloid species among the nurseries. Seedling age affected the relative abundance of ECM species to some extent. Principal component analysis PCA revealed a lack of apparent correlations between the relative abundances of species and edaphic factors. Factors that may contribute to the maintenance of ECM community structure are discussed. forest nursery mycorrhiza ITS-RFLP Scots pine Résumé - Associations mycorhiziennes des semis de pin sylvestre Pinus sylvestris L. en pépinière en Pologne. Nous avons .
