Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp Original article đề tài: New insights in the recognition of the European ash species Fraxinus excelsior L. and Fraxinus angustifolia Vahl as useful tools for forest management. | Ann. For. Sci. 63 2006 733-738 INRA EDP Sciences 2006 DOI forest 2006054 733 Original article New insights in the recognition of the European ash species Fraxinus excelsior L. and Fraxinus angustifolia Vahl as useful tools for forest management Pierre R. GÉRARDa Juan F. FERNÁNDEZ-MANJARRÉsa Paola BERTOLlNOa Jean DUFOURb Christian RAQUINa Nathalie FRASCARIA-LACOSTEa a Écologie Systématique Evolution UMR CNRS-ENGREF 8079 Bât. 360 Université Paris-Sud 91405 Orsay Cedex France b INRA Unité Amélioration Génétique et Physiologie Forestières BP 20169 Ardon 45160 Olivet France Received 11 October 2005 accepted 13 January 2006 Abstract - Common ash Fraxinus excelsior L. and narrow-leaved ash F. angustifolia Vahl are the most common ash species in Western Europe. The former is considered to be a highly valuable timber tree and contamination of its seed lots by the latter is strongly undesirable. We studied molecular physiological and morphological characteristics that can help to detect the presence of F. angustifolia at the population level either in seed and or seedling samples or in adult ash leave samples from natural or managed populations. First we developed two molecular markers which correspond to a RAPD-SCAR marker and a nuclear microsatellite-derived marker. Results indicate that these markers are almost specific to one or the other species for a set of populations sampled across Europe. Second first year dormancy was studied using germination tests without stratification. F excelsior seeds did not germinate at all while germinations were observed in samples of F. angustifolia and introgressed populations after 16 weeks. In addition F. angustifolia embryo seed length ratios at the dispersal stage were significantly higher than those from F excelsior populations. This study provides straightforward and robust tools for avoiding commercial problems of impurity of seed lots and can help forest managers to certify common ash stands. ashes RAPD-SCAR dormancy .
