Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp Original article đề tài: Fingerprinting and genetic variability in cork oak (Quercus suber L.) elite trees using ISSR and SSR markers. | Ann. For. Sci. 64 2007 773-779 INRA EDP Sciences 2007 DOI forest 2007057 Available online at Original article Fingerprinting and genetic variability in cork oak Quercus suber L. elite trees using ISSR and SSR markers Aimara LÓPEZ-ALJORNAa María Ángeles BUENOa Itziar AGUINAGALDEb Juan Pedro MARTÍNb a INIA-CIFOR Lab. Biotecnología Forestal Ctra. de La Coruna km 28040 Madrid Spain b Departamento de Biología Vegetal Escuela Técnica Superior de Ingenieros Agrónomos Universidad Politécnica de Madrid Ciudad Universitaria s n 28040 Madrid Spain Received 22 June 2006 accepted 13 March 2007 Abstract - Quercus suber L. is a socially economically and ecologically important forest species in rural areas of the Mediterranean basin. Fifty three elite-trees from nine stands of four provenance regions in the Community of Extremadura Spain were analysed with the aim to establish their DNA-fingerprinting and the genetic relationships among them. Two types of molecular markers microsatellites and intermicrosatellites were used for tree genotyping. Microsatellite markers could distinguish 94 of the trees. Observed and expected heterozygosities and effective number of alleles were correlated with the polymorphism information content PIC . Intermicrosatellite patterns were used to construct a dendrogram. They showed high levels of genetic diversity in these elite trees without any clear relationship with provenance regions. Both types of markers detected a high level of intra-stand variability which is common in Quercus species probably due to its mating system the low distance among stands and the small size of stands. Others factors that could affect this result such as introgression between Q. suber L. and Q. ilex L. are also commented. The high level of genetic variability detected in these elite trees can be useful for improvement programs. Also the usefulness of SSR and ISSR markers to establish the DNA-fingerprinting of each tree could be focused to
