Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Characterization of Antigenic Sites on the Rinderpest Virus N Protein Using Monoclonal Antibodies | J. Vet. Sci. 2003 4 1 57-65 JOURNAL OF Veterinary Science Characterization of Antigenic Sites on the Rinderpest Virus N Protein Uusing Monoclonal Antibodies Kang-seuk Choi Jin-ju Nah Young-joon Ko Cheong-up Choi Jae-hong Kim Shien-young Kang1 and Yi-seok Joo National Veterinary Research and Quarantine service Ministry of Agriculture and Forestry 480 Anyang Kyounggi 430-824 Korea IResearch Institute of Veterinary Medicine College of Veterinary Medicine Chungbuk National University 48 Gaeshin-dong Heungduk-gu Cheongju Chungbuk 361-763 Received March 4 2003 Accepted April 10 2003 Korea Abstract The N protein of the rinderpest virus RPV was analyzed topologically and antigenically by using anti-N monoclonal antibodies Mabs . Ten Mabs were raised against the N protein of the RPV. At least six non-overlapping antigenic sites sites A-F were delineated by com petitive binding assays using biotinylated Mabs. Of them 5 sites A C D E and F on the N protein were recognized by RPV-specific Mabs in ELISA and IFA while site B was recognized by Mabs reacting with both RPV and PPRV. Nonreciprocal competition w as found among sites C D and E. Recombinant RPV N protein after exposure to SDS exhibited higher ELISA titers in all Mabs recognizing 6 sites. Four sites A B E and F on 2 SDS-treated N protein lost com pletely re activity with Mabs w hile the rem aining sites C and D on the protein retained their antigenicity to some degree. It indicates that two sites C and D were sequential. Six representative Mabs bound to each site exhibited com petition with rinderpest antibodies in a blocking ELISA indicating that the sites were actively involved in antigenicity in c attle. Key words monoclonal antibody N protein rinderpest virus antigenicity Introduction Rin der pest viru s RPV has caused an acut e febr ile and highly contagious disease in cattle and wild bovids in Afr ica the Middle East and South Asia for sever al decades. Recent ly Corresponding author Kang-seuk Choi National .
