Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Activation of embryonic intermediate filaments contributes to glial scar formation after spinal cord injury in rats | J. Vet. Sei. 2003 4 2 109-112 JOURNAL OF Veterinary Science Activation of Embryonic Intermediate Filaments Contributes to Glial Scar Formation after Spinal Cord Injury in Rats Do-hyun Kim Seung-dam Heo Mee-jung Ahn Ki-bum Sim1 and Tae-kyun Shin Department of Veterinary Medicine Jeju National University Jeju 690-756 Korea Department of Neurosurgery Cheju National University Jeju 690-756 Korea Received May 18 2003 Accepted July 30 2003 Abstract The expression of two intermediate filaments nestin and vimentin was studied in spinal cord injury SCI to elucidate their roles in the formation of glial scars. Rats were sacrificed 1 4 and 7 days after induction of compression injury of the spinal cord using an aneurysm clip. The affected spinal cords were studied using antibodies against nestin and vimentin intermediate filaments. One day after spinal cord injury some clusters of nestin-positive vessels were detected in the center of the injury but few were seen in other cell types. Vimentin immunostaining was detected in some glial cells in the center and its level of immunoreactivity was enhanced in the ependymal cells of the central canal. On days 4 and 7 after spinal cord injury astrocytes and some ependymal cells in the central canal were stained positively for nestin and increased expression of nestin was observed in vessels. Vimentin was detected in some macrophages and astrocytes in the lesions. Nestin was co-localized with glial fibrillary acidic protein in some glial cells in SCI. These findings imply that spinal cord cells in adult animals have embryonic capacity and these cells are activated after injury which in turn contributes to repair of spinal cord injury through formation of a glial scar. Key words nestin spinal cord injury vimentin Introduction Spinal cord injury SCI has been postulated to include primary mechanical injury and secondary damage induced Corresponding author Tae-kyun Shin Department of Veterinary Medicine Cheju National University Jeju .
