Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Immunomodulatory and antitumor effects in vivo by the cytoplasmic fraction of Lactobacillus casei and Bifidobacterium longum | J. Vet. Sci. 2004 5 1 41-48 JOURNAL OF Veterinary Science Immunomodulatory and antitumor effects in vivo by the cytoplasmic fraction of Lactobacillus casei and Bifidobacterium longum Jung-Woo Lee Jung-Gul Shin1 Eun Hee Kim Hae Eun Kang In Been Yim Ji Yeon Kim2 Hong-Gu Joo3 and Hee Jong Woo Laboratory of Immunology College of Veterinary Medicine Seoul National University Seoul 151-742 Korea iR D Center Korea Yakult Co. LTD. Yongin 449-901 Korea Biologics Evaluation Department Safety Evaluation office Korea Food Drug Administration Seoul 122-704 Korea Department of Veterinary Medicine Cheju National University Jeju 690-756 Korea The immunomodulatory and antitumor effects of lactic acid bacteria LABs were investigated. Cytoplasmic fraction of Lactobacillus acidophilus Lactobacillus casei and Bifidobacterium longum were tested for the antiproliferative activity in vitro to SNUC2A SNU1 NIH 3T3 and Jurkat cell lines by crystal violet assay. All cytoplasmic fraction suppressed proliferation of tumor cells though L. casei and B. longum were more effective. From these results cytoplasmic fraction of L. casei and B. longum with Y400 as a control were administered as dietary supplements to Balb c mice for 2 and 4 consecutive wks. Administration for 4 wks enhanced the number of total T cells NK cells and MHC class II cells and CD4-CD8 T cells in flow cytometry analysis. To determine of antitumor activity of LABs preparation in vivo F9 teratocarcinoma cells were inoculated on mice at 14th day. Body weight was decreased with increased survival rate in all groups with the cytoplasm of LABs. Our results showed that cytoplasmic fraction of LABs had direct antiproliferative effects on tumor cell lines in vitro effects on immune cells in vivo and antitumor effects on tumor-bearing mice with prolonged survival periods. Key words Lactobacillus Bifidobacterium immunophenotyping in vivo survival rate Introduction The enhancement of the gut mucosal barrier may prevent the invasion of .
