Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Molecular fingerprinting of clinical isolates of Mycobacterium bovis and Mycobacterium tuberculosis from India by restriction fragment length polymorphism (RFLP) | J. Vet. Sci. 2004 5 4 331-335 JOURNAL OF Veterinary Science Molecular fingerprinting of clinical isolates of Mycobacterium bovis and Mycobacterium tuberculosis from India by restriction fragment length polymorphism RFLP Sandeep Kumar Singh2 Rishendra Verma1 Devendra H. Shah3 Mycobacteria Laboratory Indian Veterinary Research Institute Izatnagar-243122 . India Department of Veterinary Public Health College of Veterinary Science and Animal Husbandry G. B. Pant University of Agriculture and Technology Pantnagar 263145 . India Biosafety Research Institute Department of Veterinary Internal Medicine Teaching Veterinary Hospital College of Veterinary Medicine Chonbuk National University Jeonju 561-756 Korea Forty mycobacterial strains comprising clinical Indian isolates of Mycobacterium tuberculosis 28 field isolates 1H37 Rv and Mycobacterium bovis 10 field isolates 1 AN5 were subjected to restriction fragment length polymorphism analysis RFLP using IS6110 and IS1081 probes. Most of these strains originated from dairy cattle herd and human patients from Indian Veterinary research Institute IVRI campus isolated from the period of 1986 to 2000. Our study showed presence of 8 copies of IS6110 in most of the strains irrespective of their origin with the exception of one strain with presence of an extra copy . All strains showed a single copy of IS6110 on the characteristic restriction fragment. RFLP analysis with IS1081 invariably showed the presence of 5 copies in all isolates of and at the same chromosomal location. Similarity of IS6110 RFLP fingerprints of strains from animals and human suggested the possibility of dissemination of single strain among animals as well as human. It was not possible to discriminate within the isolates of either or when IS1081 was used as target sequence. The IS6110 RFLP is a valuable tool for disclosing .
