Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Identification and antigenic site analysis of foot-and-mouth disease virus from pigs and cattle in Korea | J. Vet. Sci. 2005 6 2 117-124 JOURNAL OF Veterinary Science Identification and antigenic site analysis of foot-and-mouth disease virus from pigs and cattle in Korea Jae Ku Oem Kwang Nyeong Lee1 In Soo Cho1 Soo Jeong Kye1 Jee Yong Park Jong Hyeon Park 7 CT VCT 7 7 CT V 7 CT 7 CT 7 Yong Joo Kim Yi Seok Joo1 Hee Jong Song2 National Veterinary Research and Quarantine Service Ministry of Agriculture and Forestry Anyang 430-824 Korea 2Department of Veterinary Infectious Disease College of Veterinary Medicine Chonbuk National University Jeonju 561-756 Korea From May to June 2002 a total of 16 foot-and mouth disease FMD outbreaks due to the serotype O virus Pan Asia strain were recorded in Korea. The viruses were identified by antigen ELISA RT-PCR and sequence analysis. The overall nucleotide sequence divergence of the VP1 region among the 4 isolates in 2002 was 0 to but between O SKR 2002 and O SKR 2000 isolates was . Phylogenetic analysis with the some known strains from East Asian countries showed that the 4 Korean isolates in 2002 formed one distinct cluster which different from clusters of Korean isolates in 2000 with in the same lineage of the ME-SA topotype strains. Deduced amino acid sequences around neutralizable antigenic site on VP1 site of O SKR 2002 isolates were aligned and compared with other strains. At the antigenic site 1 the replacements of the critical amino acid residues at position 144 from V to L and at position 152 from A to T were observed in O SKR 2002 viruses. For antigenic site 2 and 4 there were not significant variations in general. At the antigenic site 3 the substitutions of amino acid residues were present at positions 54 and 56 in O SKR 2002 isolates and an alternative residue I at position 54 are observed only at the sequence of O SKR AS 2002 cow virus. And the substitution L P of significant residue at position 144 was detected at the amino acid sequence of the O SKR 2002 cow virus. Key words antigenic site foot-and-mouth .
