Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Apoptosis in Vero cells infected with Akabane, Aino and Chuzan virus | J. Vet. Set. 2005 6 3 251-254 JOURNAL OF Short Communication Veterinary Science Apoptosis in Vero cells infected with Akabane Aino and Chuzan virus Seong In Lim Chang Hee Kweon Dong Kun Yang Dong Seob Tark Jun Hun Kweon National Veterinary Research and Quarantine Service Ministry of Agriculture and Forestry Anyang 430-824 Korea Akabane Aino and Chuzan vứus are arthropod-borne arbo viruses mainly associated with reproductive failures in cattle. We investigated apoptosis in Vero cells C-1586 infected with Akabane Aino and Chuzan virus. The fragmentation of chromosomal DNA was simultaneously detected with the progress of cytopathic effect from 48 hr to 72 hr post infection depending on viruses. Although the treatment of cycloheximide blocked apoptosis in Vero cells infected with three viruses actinomycin D did not prevent DNA oligomerization thus indicating that de novo viral protein synthesis is critical for viral apoptosis. In addition the activation of caspase-3 was also detected in Vero cells by indirect fluorescent assay. From the present results it is of future interest whether apoptotic characteristics of these viruses are related to pathogenecity in vivo. Key words Aino virus Akabane virus Chuzan virus apoptosis Vero cells Akabane Aino and Chuzan virus are arthropod-borne arbo viruses transmitted by blood-sucking insects like mosquitoes midges and ticks. Arbo viruses are a diverse group of RNA viruses that replicate in hematophagous arthropods vectors prior to transmission to human or animal host. Medically important arboviruses are classified within the families of Togaviridae Flaviviridae and Bunyaviridae 19 . Akabane and Aino virus are Bunyaviruses in the family of Bunyaviridae and Chuzan virus is Orbivirus in the family of Reoviridae. Three arbovirus infections are mainly associated with abortions stillbirths and congenital defects in pregnant cattle sheep and goat. The incidence of these viruses is widely distributed in Southest Asia and Australia 1 15 17
