Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Primary survey of avian influenza virus and Newcastle disease virus infection in wild birds in some areas of Heilongjiang Province, China | J. Vet. Set. 2005 6 4 311-315 JOURNAL OF Veterinary Science Primary survey of avian influenza virus and Newcastle disease virus infection in wild birds in some areas of Heilongjiang Province China Yu-Ping Hua Hong-Liang Chai Si-Yuan Yang Xiang-Wei Zeng Ying Sun College of Wildlife Resources Northeast Forestry University Harbin 150040 p. R. China Two hundred thứty specimens of wild birds were collected from some areas in Heilongjiang Province during the period of 2003 2004 including two batches of specimens collected randomly from a same flock of mallards in Zhalong Natural Reserve in August and December 2004 respectively. Primary virus isolation and identification for avian influenza virus AIV and Newcastle disease virus NDV were performed. The results showed that only two specimens of young mallards collected from Zhalong Natural Reserve in August 2004 were positive to AIV isolation rate and one strain D57 of these two virus isolates was identified to be H9 subtype by hemagglutination inhibition test. Meanwhile the two batches of blood serum samples of mallards from Zhalong were also examined for antibodies against AIV and NDV. Among 38 blood serum samples collected in August antibodies against the hemagglutinin of Hl H3 H5 H6 and H9 subtypes of AIV were found in 1 0 2 0 and 8 samples respectively and 11 samples were found with antibody against NDV. Whereas the NDV isolation in both two batches of specimens of mallard was negative all of the 32 blood serum samples collected in December were negative for antibodies against AIV and NDV. Key words AIV China Heilongjing Province NDV wild birds Introduction The virus causing avian influenza belongs family Orthomyxoviridae. The virus could be divided into several subtypes based on the antigenic relationships of the virus glycoproteins hemagglutinin H and neuraminidase N . Presently 15 H subtypes H1-H15 and 9 neuraminidase subtypes N1-N9 have been recognized 1 . Newcastle disease virus NDV is a member of Rubulavirus
