Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Effect of Fasciola gigantica excretory secretory antigen on rat hematological indices | J. Vet. Sci. 2006 7 2 123-125 JOURNAL OF Veterinary Science Effect of Fasciola gigantica excretory secretory antigen on rat hematological indices G Ganga1 J. p. Varshney2 R. L. Sharma3 1 Clinical Medicine Laboratory 2Division of Medicine and3Division of Parasitology Indian Veterinary Research Institute Izatnagar Bareilly-243 122 . India The present study was undertaken to investigate the effect of Fasciola gigantica excretory secretory antigen Fg-ESA on rat hematological indices. Fg-ESA was prepared by keeping thoroughly washed 40 E gigantica flukes in 100 ml phosphate buffer saline PBS for 2 h at 37 c and centrifuging the supernatant at 12 000 g at 4 C for 30 min. The protein content of Fg-ESA was adjusted to mg ml. The rats were randomly divided into two groups of six rats each. Rats in group A received ml of Fg-ESA intraperitoneally . for 7 days whereas control rats in group B received ml of PBS . for 7 days. Hemograms of both groups were studied initially and on days 0 2 4 14 and 21 after the final injection of Fg-ESA or PBS. Progressive and significant Ip declines in the values of hemoglobin hematocrit and total erythrocyte count were observed without significant Ip changes in the values of mean corpuscular hemoglobin mean corpuscular hemoglobin concentration or mean corpuscular volume in group A. Thus we conclude that Fg-ESA induces normocytic normochromic anemia in rats. Key words excretory secretory antigen Fasciola gigantica hematocrit hemoglobin rat total erythrocyte count Introduction Anemia and hypoalbuminemia are the most common and consistent accompaniments of chronic fasciolosis. However the cause of anemia in fasciolosis remains to be determined. It is not known whether the parasite is hematophagus or a tissue dweller or whether the anemia is induced by the metabolites discharged by the parasite in situ or to severe damage to the liver parenchyma and consequent hemorrhage. Levels of the interleukins IL-6 and IL-8 have
