Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Evaluation of a Bacillus stearothermophilus tube test as a screening tool for anticoccidial residues in poultry | J. Vet. Set. 2006 7 2 181-187 JOURNAL OF Veterinary Science Random amplified polymorphic DNA RAPD analysis of Mycobacterium tuberculosis strains in India J. p. N. Singh1 Rishendra Verma1 p. Chaudhuri2 1 Division of Bacteriology and Mycology Indian Veterinary Research Institute Izatnagar-243122 India division Veterinary Biotechnology Indian Veterinary Research Institute Izatnagar-243122 . India The usefulness of random amplification of polymorphic DNA RAPD analysis for typing Indian strains of M. tuberculosis was investigated. M. tuberculosis H37Rv M. tuberculosis DT and 42 clinical isolates of M. tuberculosis were subjected to RAPD-PCR using 7 random decamer primers. All 7 primers were found to be differentiated and produced specific RAPD profiles. The polymorphic amplicons served as RAPD markers for M. tuberculosis. The dendrograms obtained by different primers showed the discriminatory ability of the primers. RAPD analysis provided a rapid and easy means of identifying polymorphism in M. tuberculosis isolates and it was found to be a valuable alternative epidemiological tool. In addition the results of the present study showed heterogeneity in the M. tuberculosis strains in the population studied. Key words Mycobacterium tuberculosis RAPD typing Introduction The Mycobacterium tuberculosis complex group includes M. tuberculosis M. bovis M. africanum M. microti 19 and a newly described species M. Canetti 24 Mycobacterium tuberculosis is the primary causative agent of human tuberculosis but may also infect animals in contact with infected humans 15 . Tuberculosis has re-emerged as one of the leading causes of death worldwide causing nearly three million deaths annually 2 In India alone half a million people die of TB every year . more than 1000 people every day and one patient every minute 29 . Both M. tuberculosis and M. bovis have been isolated from humans and animals in India 26 . However the origin and transmission of infection between animals and .
