Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Enhanced immune response with foot and mouth disease virus VP1 and interleukin-1 fusion genes | J. Vet. Set. 2006 7 3 257-262 JOURNAL OF Veterinary Science Enhanced immune response with foot and mouth disease virus VP1 and interleukin-1 fusion genes Jong Hyeon Park Sun Jin Kim Jae Ku Oem Kwang Nyeong Lee Yong Joo Kim Soo Jeong Kye Jee Yong Park Yi Seok Joo National Veterinary Research and Quarantine Service Ministry of Agriculture and Forestry Anyang 430-824 Korea The capsid of the foot and mouth disease FMD virus carries the epitopes that are critical for inducing the immune response. In an attempt to enhance the specific immune response plasmid DNA was constructed to express VPl interleukin-la IL-la and precursor capsid Pl in combination with 2A Pl-2A IL-la under the control of the human cytomegalovirus HCMV immediate-early promoter and intron. After DNA transfection into MA 104 monkey kidney cells Western blotting and an immunofluorescence assay were used to confirm the expression of VP I or P1-2A and IL-la. Mice were inoculated with the encoding plasmids via the intradermal route and the IgGl and IgG2a levels were used to determine the immune responses. These results show that although the immunized groups did not carry a high level of neutralizing antibodies the plasmids encoding the VP1 IL-la and P1-2A IL-la fused genes were effective in inducing an enhanced immune response. Key words foot and mouth disease immunogenicity interleukin-1 Introduction Foot and mouth disease FMD is one of the most devastating diseases in cattle swine sheep goats and many wild cloven-hoofed animal species 2 The causative agent of the disease belongs to the Aphthoviruses genus of the Picornaviridae Family. In recent years DNA vaccination has become one of the most promising routes for a recombinant vaccine 8 12 allowing a safe and efficient alternative to conventional vaccination. DNA vaccine technology facilitates the use of cytokines as modulators in vaccination to manipulate the immune responses. In particular IL-1 production by Corresponding author Tel 82-31-467-1719 Fax
