Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Production of monoclonal antibodies against serum immunoglobulins of black rockfish (Sebastes schlegeli Higendorf) | J. Vet. Set. 2006 7 3 293-295 JOURNAL OF Short Communication Veterinary Science Production of monoclonal antibodies against serum immunoglobulins of black rockfish Sebastes schlegeli Higendorf Geewook Shin1 Hyungiun Lee1 K. J. Palaksha1 Youngrim Kim1 Eunyoung Lee1 Yongseung Shin1 Eunggoo Lee1 Kyungdae Park2 Taesung Jung1 Laboratory of Fish Shellfish Diseases College of Veterinary Medicine Gyeongsang National University Jinju 660-701 Korea 2Gyeongsangnam-do Fisheries Resources Research Institute Tongyeong 650-947 Korea The present study was undertaken to produce monoclonal antibodies MAbs against immunoglobulin Ig purified from black rockfish Sebastes schlegeli Higendorf serum using protein A mannan binding protein and goat IgG affinity columns. These three different ligands were found to possess high affinity for black rockfish serum Ig. All of the Igs purified eluted at only M NaCl concentration in anion exchange column chromatography and consisted of two bands at 70 kDa and 25 kDa in SDS-PAGE they also had similar antigenicity for MAbs to Ig heavy chain in immunoblot assays. Therefore black rockfish Ig is believed to exist as a single isotype within serum. The MAbs produced against Ig heavy chain reacted specifically with spots distributed over the pl range from to with a molecular weight of 70kDa on two dimensional gel electrophoresis immunoblot profiles. Key words black rockfish immunoglobulin monoclonal antibodies Sebastes schlegeli Higendorf The defense mechanisms of vertebrates are generally divided into two types the innate and acqufred immune responses. Acquired immune responses involve the production of specific immunoglobulins Igs by the host following antigen stimulation 19 . The Igs produced protect the host by removing the pathogen or preventing it from spreading into the blood or organs. Mammals possess five classes of Ig heavy chains IgM IgG IgA IgE and IgD 19 . Fish on the other hand have been reported to have four Ig heavy chain .
