Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Cellular uptake of magnetic nanoparticle is mediated through energy-dependent endocytosis in A549 cells | J. Vet. Set. 2006 7 4 321-326 JOURNAL OF Veterinary Science Cellular uptake of magnetic nanoparticle is mediated through energydependent endocytosis in À549 cells Jun-Sung Kim1 Tae-Jong Yoon2 Kyeong-Nam Yu1 Mi Suk Noh1 Minah Woo1 Byung-Geol Kim2 Kee-Ho Lee3 Byung-Hvuk Sohn4 Seung-Bum Park5 Jin-Kyu Lee2 Myung-Haing Cho1 Laboratory of Toxicology College of Veterinary Medicine 2 Materials Chemistry Lab 4Polymeric and Soft Nanomaterials 5Diversity Oriented Synthesis and Chemical Biology Lab Seoul National University Seoul 151-742 Korea 3Laboratory of Molecular Oncology Korea Institute of Radiological and Medical Sciences Seoul 139-706 Korea Biocompatible silica-overcoated magnetic nanoparticles containing an organic fluorescence dye rhodamine B isothiocyanate RITC within a silica shell 50 nm size MNP@SiO2 RITC s were synthesized. For future application of the MNP@SiO2 RITC s into diverse areas of research such as drug or gene delivery bioimaging and biosensors detailed information of the cellular uptake process of the nanoparticles is essential. Thus this study was performed to elucidate the precise mechanism by which the lung cancer cells uptake the magnetic nanoparticles. Lung cells were chosen for this study because inhalation is the most likely route of exposure and lung cancer cells were also found to uptake magnetic nanoparticles rapidly in preliminary experiments. The lung cells were pretreated with different metabolic inhibitors. Our results revealed that low temperature disturbed the uptake of magnetic nanoparticles into the cells. Metabolic inhibitors also prevented the delivery of the materials into cells. Use of TEM clearly demonstrated that uptake of the nanoparticles was mediated through endosomes. Taken together our results demonstrate that magnetic nanoparticles can be internalized into the cells through an energy-dependent endosomal-lysosomal mechanism. Key words A549 cells cellular uptake endocytosis magnetic nanoparticle Introduction Nanoparticles .
