Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: In vitro neuronal and osteogenic differentiation of mesenchymal stem cells from human umbilical cord blood | J. Vet. Set. 2006 7 4 343-348 JOURNAL OF Veterinary Science In vitro neuronal and osteogenic differentiation of mesenchymal stem cells from human umbilical cord blood Ki-Soo Park1 Yong-Soon Lee1 Kyung-Sun Kang1A Laboratory of Stem cell and Tumor Biology Department of Veterinary Public Health and2Adult Stem Cell Research Center College of Veterinary Medicine Seoul National University Seoul 151-742 Korea Mesenchymal stem cells MSCs have the capabilities for self-renewal and differentiation into cells with the phenotypes of bone cartilage neurons and fat cells. These features of MSCs have attracted the attention of investigators for using MSCs for cell-based therapies to treat several human diseases. Because bone marrow-derived cells which are a main source of MSCs are not always acceptable due to a significant drop in their cell number and proliferative differentiation capacity with age human umbilical cord blood UCB cells are good substitutes for BMCs due to the immaturity of newborn cells. Although the isolation of hematopoietic stem cells from UCB has been well established the isolation and characterization of MSCs from UCB still need to be established and evaluated. In this study we isolated and characterized MSCs. UCB-derived mononuclear cells which gave rise to adherent cells exhibited either an osteoclast or a mesenchymal-like phenotype. The attached cells with mesenchymal phenotypes displayed fibroblast-like morphologies and they expressed mesenchym-related antigens SH2 and vimentin and periodic acid Schiff activity. Also UCB-derived MSCs were able to transdifferentiate into bone and 2 types of neuronal cells in vitro. Therefore it is suggested that the MSCs from UCB might be a good alternative to bone marrow cells for transplantation or cell therapy. Key words differentiation human mesenchymal cell stem cell umbilical cord blood Introduction The blood remaining in the umbilical cord following birth contains hematopoietic precursors and this has become an .
