Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Birth of puppies after intrauterine and intratubal insemination with frozen-thawed canine semen | J. Vet. Set. 2007 8 1 75-80 JOURNAL OF Veterinary Science Birth of puppies after intrauterine and intratubal insemination with frozen-thawed canine semen Hye Jin Kim1 3 Hyun Ju Oh3 Goo Jang3 Min Kyu Kim23 iHaemaru Small Animal Referral Hospital SungNam 463-050 Korea 2Veterinary Medical Teaching Hospital and3Department ofTheriogenology and Biotechnology College of Veterinary Medicine Seoul National University Seoul 151-742 Korea The present study was performed to assess the fertility of frozen-thawed dog semen prepared by freezing with 6 glycerol and thawing at 70 C for 8 sec and to evaluate the least number of post-thaw spermatozoa necessary to achieve pregnancy by intrauterine or intratubal artificial insemination. It was found that the pregnancy rate of intrauterine artificial insemination was 100 using 6 glycerol buffer and thawing at 70 C for 8 sec with 5 X 107 spermatozoa. Even though the pregnancy rate 80 and the whelping rate in the 5 X 106 spermatozoa inseminated group were lower than those of the 5 X 107 spermatozoa group conception was confirmed with 5 X 106 spermatozoa. Although the pregnancy rate of intratubal insemination was low 20 with 4 X 106 spermatozoa this study is the first report to show the pregnancy rate of intratubal insemination with frozen-thawed ejaculated canine semen. In order to improve the pregnancy rate with intratubal insemination of canine spermatozoa it is necessary to investigate the optimal insemination site of the uterine tube the appropriate number of sperm and the direct effect of buffer on oocytes. Key words frozen dog semen glycerol intratubal insemination intrauterine insemination thawing temperature Introduction Artificial insemination is considered to be an in vivo assay of spermatozoa survival and is the most definitive test of sperm function. Artificial insemination with fresh or frozen-thawed ejaculated spermatozoa has been used widely and successfully in canines 4 5 11 12 15 18 . Various methods of insemination
