Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Immunohistochemical expression of h-telomerase reverse transcriptase in canine and feline meningiomas | J. Vet. Set. 2007 8 2 111-115 JOURNAL OF Veterinary Science Immunohistochemical expression of h-telomerase reverse transcriptase in canine and feline meningiomas Luciana Mandrioli1 Serena Panarese1 Alessandro Cesari1 Maria Teresa Mandara2 Paolo Stefano Marcato1 Giuliano Bettini1 1 Department of Veterinary Public Health and Animal Pathology Faculty of Veterinary Medicine Alma Mater Studiorum-University of Bologna Italy Department ofBiopathological Science and Hygiene of Animal and Food Production School of Veterinary Medicine Perugia Italy Telomere length maintenance is regarded as a fundamental step in tumorigenesis as most human brain tumors including meningiomas stabilize the ends of theứ chromosomes using telomerase. This investigation represents an introduction to telomerase expression in canine and feline meningiomas. Twenty-five archived cases 14 dogs and 11 cats were immunohistochemically tested for human-telomerase reverse transcriptase h-TERT scored and quantified furthermore mitoses were counted on sections stained with a modified toluidine blue. The h-TERT antibody immunolabelled the nucleus and nucleolus of meningeal neoplastic cells with an intensity ranging from mild to strong and a speckled distribution a significantly higher expression in cats was noted while no significant association between h-TERT immunolabelling and sex or histotype was evident in dogs or cats. The telomerase enzyme represents a fundamental parameter of potential malignant transformation which may occur independently of the signal to proliferate thereby supplying the cells with unlimited growth capabilities. Telomerase expression could be a prognostic indicator independent of the kinetic parameters although this should be evaluated using a larger dataset with available clinical information. Key words cat dog h-telomerase reverse transcriptase immunohistochemistry meningioma Introduction Telomerase is a ribonucleoprotein complex that prevents the erosion of chromosomal .
