Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Purification and characterization of two larval glycoproteins from the cattle tick, Boophilus annulatus | J. Vet. Set. 2007 8 2 175-180 JOURNAL OF Veterinary Science Purification and characterization of two larval glycoproteins from the cattle tick Boophilus annulatus Amr E. El Hakim1 Yasser E. Shahein Amira M. K. Abouelella2 Mohamed E. Selim3 1 Molecular Biology Department National Research Centre Cairo Egypt 2Radiation Biology Department NCRRT Cairo Egypt 3Animal Etealth Department Desert Research Centre Mataria Cairo Egypt The present study was conducted to identify new target immunogenic molecules from the larval stage of the cattle tick Boophilus annulatus Acari Ixodidae . Two specific larval glycoproteins GLPs were isolated by two-step affinity chromatography. The larval immunogens were first purified with CNBr-Sepharose coupled to rabbit anti-larval immunoglobulins and the glycoproteins were then purified with Con-A Sepharose. These glycoproteins have molecular weights of approximately 32 and 15 kDa with isoelectric points between and . Antibodies against the two GLPs labeled I and II were detected in the anti-whole tick -whole larval and -gut antigens through immunoblot analysis. These results suggest that these GLPs are good immunogens and can be useful in the vaccination of cattle against tick infestation. Key words Boophilus annulatus glycoproteins larvae vaccination. Introduction Ticks are of great veterinary importance compared to other ectoparasites. They consume large quantities of host blood during theứ lengthy attachment period 7-14 days which may be extended depending on the tick species and unique host association. The bovine tick Boophilus annulatus B. annulatus is a bloodsucking ectoparasite that causes severe production losses in the cattle industry. The average tick burden causes an annual weight loss of kg tick. With the huge number of ticks infesting cattle and other livestock animals the subsequent effect on beef production is a reduction of hundreds of millions of kilograms annually. Camels cattle and chickens are severely affected
