Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: The expression and cellular localization of phospholipase Disozymes in the developing mouse testis | J. Vet. Set. 2007 8 3 209-212 JOURNAL OF Veterinary Science The expression and cellular localization of phospholipase D isozymes in the developing mouse testis Heechul Kim 2 Yongduk Lee1 Jin Won Hyun23 Young Ho Lee4 Min Kyoung Shin5 7 z cs z V z cs z V cs z Do Sik Min5 Taekyun Shinu 1 Department of Veterinary Medicine College of Applied Life Sciences 2Applied Radiological Science Research Institute and 3Department of Biochemistry College of Medicine Cheju National University Jeju 690-756 Korea 4Department of Anatomy College of Medicine Chungnam National University Daejeon 301-131 Korea 5Department of Molecular Biology College of Natural Science Pusan National University Busan 609-735 Korea To examine the involvement of phospholipase D PLD isozymes in postnatal testis development the expression of PLD1 and PLD2 was examined in the mouse testis at postnatal weeks 1 2 4 and 8 using Western blot analysis and immunohistochemistry. The expression of both PLD1 and PLD2 increased gradually with development from postnatal week 1 to 8. Immunohistochemically PLD immunoreactivity was detected in some germ cells in the testis and interstitial Leydig cells at postnatal week 1. PLD was mainly detected in the spermatocytes and residual bodies of spermatids in the testis after 8 weeks after birth. The intense immunostaining of PLD in Leydig cells remained unchanged by postnatal week 8. These findings suggest that PLD isozymes are involved in the spermatogenesis of the mouse testis. Key words cellular localization mouse phospholipase D spermatogenesis testis Introduction Phospholipase D PLD which catalyzes the hydrolysis of phosphatidylcholine to phosphatidic acid PA and choline has been suggested to play an important role in receptor-mediated signal pathways leading to cell proliferation differentiation apoptosis differentiation cytoskeletal reorganization and membrane trafficking and secretory events possibly including neurotransmitter release 4-6 . PA is generally recognized as .
