Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Effects of endocrine disrupting chemicals on expression of phospholipid hydroperoxide glutathione peroxidase mRNA in rat testes | J. Vet. Set. 2007 8 3 213-218 JOURNAL OF Veterinary Science Effects of endocrine disrupting chemicals on expression of phospholipid hydroperoxide glutathione peroxidase mRNA in rat testes T T - 1 1 T 1 -XT 1 T Ixr T 1 1 r T7 1 T- Al 1 T m T W Ĩ In-Jeoung Baek Jung-Min Yon Se-Ra Lee Yan Jin Mi-Ra Kim Byeongwoo Ahn Jin Tae Hong Young-Kug Choo3 Beom Jun Lee1 Young Won Yun1 Sang-Yoon Nam1 1 College of Veterinary Medicine and Research Institute of Veterinary Medicine and 2 College of Pharmacy Chungbuk National University Cheongju 361-763 Korea iDepartment of Biological Science College of Natural Sciences Wonkwang University Iksan 570-749 Korea Phospholipid hydroperoxide glutathione peroxidase PHGPx an antioxidative selenoprotein is modulated by estrogen in the testis and oviduct. To examine whether potential endocrine disrupting chemicals EDCs affect the microenvironment of the testes the expression patterns of PHGPx tnRNA and histological changes were analyzed in 5-week-old Sprague-Dawley male rats exposed to several EDCs such as an androgenic compound testosterone 50 200 and 1 000 Ltg kg anti-androgenic compounds flutamide 1 5 and 25 mg kg ketoconazole and 1 mg kg and diethylhexyl phthalate 10 50 and 250mg kg J and estrogenic compounds nonylphenol 10 50 100 and 250 mg kg octylphenol 10 50 and 250 mg kg and diethylstilbestrol 10 20 and 40 pg kg daily for 3 weeks via oral administration. Mild proliferation of germ cells and hyperplasia of interstitial cells were observed in the testes of the flutamide-treated group and deletion of the germinal epithelium and sloughing of germ cells were observed in testes of the diethylstilbestrol-treated group. Treatment with testosterone was shown to slightly decrease PHGPx mRNA levels in testes by the reverse transcription-polymerase chain reaction. However anti-androgenic compounds flutamide ketoconazole and diethylhexyl phthalate and estrogenic compounds nonylphenol octylphenol and diethylstilbestrol significantly up-regulated
