Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Increased phosphorylation of caveolin-1 in the spinal cord of irradiated rats | J. Vet. Sci. 2007 8 4 323-327 JOURNAL OF Veterinary Science Increased phosphorylation of caveolin-1 in the spinal cord of irradiated rats Heechul Kim1 2 3 t Changjong Moon4 t Jeongtae Kim1 Meejung Ahn1 2 3 Jin Won Hyun2 5 Jae Woo Park2 6 Sung-Ho Kim4 Seungjoon Kim2 Taekyun Shin1 2 3 Department of Veterinary Medicine College of Applied Life Sciences 2Applied Radiological Science Research Institute 3Research Institute for Subtropical Agriculture and Biotechnology 5Department of Biochemistry College of Medicine and 6Department of Nuclear and Energy Engineering College of Engineering Cheju National University Jeju 690-756 Korea 4Department of Veterinary Anatomy College of Veterinary Medicine Chonnam National University Gwangju 500-757 Korea Phosphorylation of caveolin-1 occurs during cell activation by various stimuli. In this study the involvement of caveolin-1 in an irradiation injured spinal cord was examined by analyzing the phosphorylation of caveolin-1 in the spinal cord of rats after irradiation with a single dose of 15 Gray from a 60Co Y-ray source at 24 h post-irradiation PI . A Western blot analysis showed that the phosphorylated form of caveolin-1 p-caveolin-1 was expressed constitutively in the normal spinal cords and was significantly higher in the spinal cord of irradiated rats at 24 h PI. The increased expression of ED1 which is a marker of activated microglia macrophages was matched with that of p-caveolin-1. In the irradiated spinal cords there was a higher level of p-caveolin-1 immunoreactivity in the isolectin B4-positive microglial ependymal and vascular endothelial cells in which p-caveolin-1 was weakly and constitutively expressed in the normal control spinal cords. These results suggest that total body irradiation induces activation of microglial cells in the spinal cord through the phosphorylation of caveolin-1. Key words caveolin-1 irradiation microglia phosphorylation spinal cord Introduction Caveolin is a transmembrane adapter molecule that .
