Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Enhanced tyrosine hydroxylase expression in PC12 cells co-cultured with feline mesenchymal stem cells | J. Vet. Sci. 2007 8 4 377-382 JOURNAL OF Veterinary Science Enhanced tyrosine hydroxylase expression in PC12 cells co-cultured with feline mesenchymal stem cells 1 4 X7--4 1 4 3 1 2 Guang-Zhen Jin Xi-Jun Yin Xian-Feng Yu Su-Jin Cho Hyo-Sang Lee Hyo-Jong Lee Il-Keun Kong Division of Applied Life Science 2Institute of Agriculture and Life Science 3College of Veterinary Medicine Gyeongsang National University Jinju 660-701 Korea 4Department of Animal Science Technology Sunchon National University Suncheon 540-742 Korea Mesenchymal stem cells MSCs secrete a variety of neu-roregulatory molecules such as nerve growth factor brain-derived neurotrophic factor and glial cell-derived neurotrophic factor which upregulate tyrosine hydroxylase TH gene expression in PC12 cells. Enhancing TH gene expression is a critical step for treatment of Parkinson s disease PD . The objective of this study was to assess the effects of co-culturing PC12 cells with MSCs from feline bone marrow on TH protein expression. We divided the study into three groups an MSC group a PC12 cell group and the combined MSC PC12 cell group the co-culture group . All cells were cultured in DMEM-HG medium supplemented with 10 fetal bovine serum for three days. Thereafter the cells were examined using western blot analysis and immunocytochemistry. In western blots the co-culture group demonstrated a stronger signal at 60 kDa than the PC12 cell group p . TH was not expressed in the MSC group either in western blot or immunocytochemistry. Thus the MSCs of feline bone marrow can up-regulate TH expression in PC12 cells. This implies a new role for MSCs in the neuro-degenerative disease process. Key words co-culture feline bone marrow mesenchymal stem cell rat PC12 cell tyrosine hydroxylase Introduction Parkinson s disease PD is characterized by a loss of dopamine in the striatum resulting from the progressive degeneration of dopaminergic neurons within the substantia nigra 20 . Tyrosine hydroxylase TH is the .
