Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Use of flow cytometry to develop and characterize a set of monoclonal antibodies specific for rabbit leukocyte differentiation molecules | J. Vet. Sci. 2008 9 1 51-66 JOURNAL OF Veterinary Science Use of flow cytometry to develop and characterize a set of monoclonal antibodies specific for rabbit leukocyte differentiation molecules William C. Davis Mary Jo Hamilton Department of Veterinary Microbiology and Pathology College of Veterinary Medicine Washington State University Pullman WA 99164-7040 USA Flow cytometry was used to identify and characterize monoclonal antibodies mAbs that react with rabbit leukocyte differentiation molecules LDM . Screening sets of mAbs developed against LDM in other species for reactivity with rabbit LDM yielded 11 mAbs that recognize conserved epitopes on rabbit LDM orthologues and multiple mAbs that recognize epitopes expressed on the major histocompatibility class I or class II molecules. Screening of mAbs submitted to the Animal Homologues Section of the Eighth Human Leukocyte Differentiation Workshop yielded 7 additional mAbs. Screening of mAbs generated from mice immunized with leukocytes from rabbit thymus or spleen or concanavalin A activated peripheral blood and or spleen lymphocytes has yielded 42 mAbs that recognize species restricted epitopes expressed on one or more lineages of leukocytes. Screening of the anti-rabbit mAbs against leukocytes from other species yielded one additional mAb. The studies show that screening of existing sets of mAbs for reactivity with rabbit LDM will not be productive and that a direct approach will be needed to develop mAbs for research in rabbits. The flow cytometric approach we developed to screen for mAbs of interest offers a way for individual laboratories to identify and characterize mAbs to LDM in rabbits and other species. A web-based program we developed provides a source of information that will facilitate analysis. It contains a searchable data base on known CD molecules and a data base on mAbs known to react with LDM in one or more species of artiodactyla equidae carnivora and or lagomorpha. Keywords leukocyte .
