Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Variable number tandem repeat analysis of Mycobacterium bovis isolates from Gyeonggi-do, Korea | J. Vet. Sci. 2008 9 2 145-153 JOURNAL OF Veterinary Science Variable number tandem repeat analysis of Mycobacterium bovis isolates from Gyeonggi-do Korea 1 _1 1-2x73 1 3 2 T Bo-Young Jeon Sungmo Je Jinhee Park Yeun Kim Eun-Gae Lee Hyeyoung Lee Sangkyo Seo Sang-Nae Cho1 4 1 Department of Microbiology and the Brain Korea 21 Project for the Medical Sciences Yonsei University College of Medicine Seoul 120-752 Korea 2Gyeonggi-do Veterinary Service Suwon 441-460 Korea 3Department of Biomedical Laboratory Sciences College of Health Sciences Yonsei University Wonju 220-710 Korea 4The International Vaccine Institute Seoul 151-600 Korea Bovine tuberculosis TB is a major zoonosis that s caused by Mycobacterium bovis M. bovis . Being able to detect M. bovis is important to control bovine TB. We applied a molecular technique the variable number tandem repeat VNTR typing method to identify and distinguish the M. bovis isolates from Gyeonggi-do Korea. From 2003 to 2004 59 M. bovis clinical strains were isolated from dairy cattle in Gyeonggi-do Korea and these cattle had tuberculosis-like lesions. Twenty-four published MIRU-VNTR markers were applied to the M. bovis isolates and ten of them showed allelic diversity. The most discriminatory locus for the M. bovis isolates in Korea was QUB 3336 h . QUB 26 and MIRU 31 also showed high discriminative power h . The allelic diversity by the combination of all VNTR loci was . Six loci MIRU 31 ETR-A and QUB-18 -26 -3232 -3336 displayed valuable allelic diversity. Twelve genotypes were identified from the 59 M. bovis isolates that originated from 20 cattle farms that were dispersed throughout the region of Gyeng-gi-do. Two genotypes designation index . e g showed the highest prevalence 20 of the total farms . For the multiple outbreaks on three farms two successive outbreaks were caused by the same genotype at two farms. Interestingly the third outbreak at one farm was caused by both a new genotype and a previous genotype.
