Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Plasma haptoglobin and immunoglobulins as diagnostic indicators of deoxynivalenol intoxication | J. Vet. Sci. 2008 9 3 257-266 JOURNAL OF Veterinary Science Plasma haptoglobin and immunoglobulins as diagnostic indicators of deoxynivalenol intoxication T1 T T7- 1 ri TT T 1. T - w yi 1 TT yxi IT- 1 TT f r 1 TT IT- 1 Eun-Joo Kim Sang-Hee Jeong Joon-Hyoung Cho Hyun-Ok Ku Hyun-Mi Pyo Hwan-Goo Kang Kyoung-Ho Choi2 National Veterinary Research Quarantine Service Anyang 430-824 Korea 2Environmental Toxicology and Risk Assessment Graduate School of Public Health Seoul National University Seoul 110-799 Korea This study aimed to discover potential biomarkers for dioxynivalenol DON intoxication. B6C3F1 male mice were orally exposed to and mg kg body weight bw DON for 8 days and the differential protein expressions in their blood plasma were determined by SELDI - Time-of-Flight Mass Spectrometry TOF MS and the immunoglobulins Igs G A M and E in the serum were investigated. kDa protein was significantly highly expressed according to DON administration and this protein was purified by employing a methyl ceramic HyperD F column with using optimization buffer for adsorption and desorption. The purified protein was identified as a haptoglobin precursor by peptide mapping with using LC Q-TOF MS and MALDI-TOF MS and this was confirmed by western blotting and ELISA. IgG and IgM in serum were decreased in a dose-dependent manner and IgA was decreased at mg kg bw DON administration but the IgE level was not changed. To compare the expressions of haptoglobin and the Igs patterns between aflatoxin B1 AFB1 zearalenone ZEA and DON intoxications rats were orally administered with AFB1 ZEA 240 and DON mg kg bw for 8 days. Haptoglobin was increased only at DON mg kg bw while it was slightly decreased at ZEA 240 mg kg bw and it was not detected at all at AFB1 mg kg bw. IgG and IgA were decreased by DON but IgG IgA IgM and IgE were all increased by AFB1. No changes were observed by ZEA administration. These results show that plasma haptoglobin could be a
