Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Distribution of the putative virulence factor encoding gene sheta in Staphylococcus hyicus strains of various origins | J. Vet. Sci. 2008 9 3 327-329 JOURNAL OF Short Communication Veterinary Science Distribution of the putative virulence factor encoding gene sheta in Staphylococcus hyicus strains of various origins 1 2 1 1. 3 Talah Kanbar Andrey V. Voytenko Jorg Alber Christoph Lammler Reinhard Weiss Vladimir N. Skvortzov1 institutfur Pharmakologie und Toxikologie Justus-Liebig-Universitat Giefien Frankfurter Str. 107 35392 Giefien Germany 2Kovalenko Research Institute of Experimental Veterinary Medicine Kurskaya Str. 4 308002 Belgorod Russia 3Institut fur Hygiene undInfektionskrankheiten der Tiere Justus-Liebig-Universitat Giefien Frankfurter Str. 85-91 35392 Giefien Germany In the present study Staphylococcus S. hyicus strains isolated in Russia n 23 and Germany n 17 were investigated for the prevalence of the previously described genes sheta and shetb. Sheta was detected in 16 S. hyicus strains. Sheta-positive strains were mainly found among strains isolated from exudative epidermitis and frequently together with the exfoliative toxin-encoding genes exhD and exhC. Partial sequencing of sheta in a single S. hyicus strain revealed an almost complete match with the sheta sequence obtained from GenBank. None of the S. hyicus strains displayed a positive reaction with the shetb-specific oligonucleotide primer used in the present study. According to the present results the exotoxin encoding gene sheta seems to be distributed among S. hyicus strains in Russia and Germany. The toxigenic potential of this exotoxin which does not have the classical structure of a staphylococcal exfoliative toxin remains to be elucidated. Keywords exfoliative toxins exudative epidermitis sheta shetb Staphylococcus hyicus Staphylococcus S. hyicus is a worldwide causative agent of exudative epidermitis in pigs a generalized infection of the skin characterized by exudation exfoliation and vesicle formation 10 . S. hyicus isolated from exudative epidermitis generally produces exfoliation-inducing exotoxins .
