Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Variation in the molecular weight of Photobacterium damselae subsp. piscicida antigens when cultured under different conditions in vitro | J. Vet. Set. 2007 8 3 255-261 JOURNAL OF Veterinary Science Variation in the molecular weight of Photobacterium damselae Allbsp. pỉscỉcỉda antigens when cultured under different conditions in vitro Tae s. Jung1 Kim D. Thompson2 Donatella Volpatti3 Marco Galeotti3 A. Adams2 1 Laboratory of Fish and Shellfish Diseases College of Veterinary Medicine Gyeongsang National University Jinju 660-701 Korea 2Aquatic Vaccine Unit Institute of Aquaculture University of Stirling Stirling FK9 4LA Scotland UK iDipartimento di Scienze della Produzione Animate Faculta Di Medicina Veterinaria Universita degli studi di Udine 20B 33100 Udine Italy The antigenicity of Photobacterium damselae Ph. d. subsp. piscicida cultured in four different growth media tryptone soya broth TSB glucose-rich medium GRM iron-depleted TSB TSB IR and ứon-depleted GRM GRM IR J was compared by enzyme-linked immunosorbent assay ELISA and Western blot analysis using sera obtained from sea bass Dicentrarchus labrax raised against live or heat-killed Ph. d. subsp. piscicida. The antigenic expression QĨPh. d. subsp. piscicida was found to differ depending on the culture medium used. A significantly higher antibody response was obtained with iron-depleted bacteria by ELISA compared with non-iron depleted bacteria obtained from the sera of sea bass raised against live Pit. d. subsp. piscicida. The sera from sea bass raised against live bacteria showed a band at 22 kDa in bacteria cultured in TSB IR or GRM IR when bacteria that had been freshly isolated from fish were used for the screening while bands at 24 and 47 kDa were observed with bacteria cultured in TSB or GRM. When bacteria were passaged several times on tryptic soya agar prior to culturing in the four different media only bands at 24 and 47 kDa were recognized regardless of the medium used to culture the bacteria. It would appear that the molecular weight of Ph. d subsp. piscicida antigens change in the presence of iron restriction and sera from sea bass .
