Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Comparative study on the aflatoxin B1 degradation ability of rumen fluid from Holstein steers and Korean native goats | J. Vet. Sci. 2009 10 1 29-34 DOI JOURNAL OF Veterinary Science Comparative study on the aflatoxin B1 degradation ability of rumen fluid from Holstein steers and Korean native goats oÀ-Ĩ. TA_ĩ. T T_11_1 TT _ z-l_o__2 - ll_TTT1 X7_1_1 o__H71 __TÍ I_3 T__ Santi Devi Upadhaya Ha Guyn Sung Chan Hee Lee Se Young Lee Sun Woo Kim Kyung Jin Cho Jong K. Ha1 Department of Agricultural Biotechnology Research Institute for Agriculture and Life Sciences College of Agriculture and Life Science Seoul National University Seoul 151-921 Korea 2Daeho Tech Hwasung 445-933 Korea 3GeneBio Tech Gongju 314-831 Korea The aflatoxin B1 degrading abilities of two different ruminants were compared in this study. One set of experiments evaluated the aflatoxin B1 degradation ability of different rumen fluid donors steers vs. goats as well as the rumen fluid filtration method cheese cloth filtered vs. pm Millipore in a 2 X 2 factorial arrangement. Additional studies examined aflatoxin B1 degradation by collecting rumen fluid at different times 0 3 6 9 and 12 h after feeding. Cannulated Holstein steers 740 10 kg bw and Korean native goats 26 3 kg bw were fed a 60 timothy and 40 commercial diet with free access to water. Rumen fluid from Korean native goats demonstrated higher p aflatoxin B1 degradability than Holstein steers. However filtration method had no significant influence on degradability. In addition aflatoxin degradation did not depend upon rumen fluid collection time after feeding as no significant differences were observed. Finally a comparison of two types of diet high in roughage found aflatoxin degradability in goats was higher with timothy hay opposed to rice straw although individual variation existed. Thus our findings showed the aflatoxin degradability is comparatively higher in goats compared to steers. Keywords aflatoxin B1 degradation ELISA rumen Introduction Aflatoxin is one of several extremely toxic .