Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Low dietary inorganic phosphate affects the lung growth of developing mice | J. Vet. Sci. 2009 10 2 105-113 DOI JOURNAL OF Veterinary Science Low dietary inorganic phosphate affects the lung growth of developing mice XT __1 1x71 1 1 1 Cheng-Xiong Xu Hua Jin Youn-Sun Chung Ji-Young Shin Soon-Kyung Hwang Jung-Taek Kwon _1 1 1 1 2 1 2 1 2 Sung-Jin Park Eun-Sun Lee Arash Minai-Tehrani Seung-Hee Chang Min-Ah Woo Mi-Suk Noh Gil-Hwan An3 Kee-Ho Lee4 Myung-Haing Cho1 2 laboratory of Toxicology College of Veterinary Medicine and 2Nano Systems Institute-National Core Research Center Seoul National University Seoul 151-742 Korea 3Department of Food Science and Technology College of Agriculture and Life Sciences Chungnam National University Daejeon 305-764 Korea 4Laboratory of Molecular Oncology Division of Radiation Cancer Research Korea Institute of Radiological and Medical Sciences Seoul 139-240 Korea Inorganic phosphate Pi plays a critical role in diverse cellular functions and regulating the Pi balance is accomplished by sodium-dependent Pi co-transporter NPT . Pulmonary NPT has recently been identified in mammalian lungs. However to date many of the studies that have involved Pi have mainly focused on its effect on bone and kidney. Therefore current study was performed to discover the potential effects of low Pi on the lung of developing transgenic mice expressing the renilla firefly luciferase dual reporter gene. Two-weeks old male mice divided into 2 groups and these groups were fed either a low PI diet or a normal control diet normal Pi low Pi for 4 weeks. After 4 weeks of the diet all the mice were sacrificed. Their lungs were harvested and analyzed by performing luciferase assay Western blotting kinase assay and immunohistochemistry. Our results demonstrate that low Pi affects the lungs of developing mice by disturbing protein translation the cell cycle and the expression of fibroblast growth factor-2. These results suggest that optimally regulating Pi consumption may be important to maintain health. .
