Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: A novel b-glucan produced by Paenibacillus polymyxa JB115 induces nitric oxide production in macrophages | J. Vet. Sci. 2009 10 2 165-167 DOI Short Communication JOURNAL OF Veterinary Science A novel p-glucan produced by Paenibacillus polymyxa JB115 induces nitric oxide production in macrophages 1 1 1 2 2 3 Zhi-Qiang Chang Joong-Su Lee Mi-Hyun Hwang Joo-Heon Hong Hee-Kyoung Jung Sam-Pin Lee Seung-Chun Park1 College of Veterinary Medicine Kyungpook National University Daegu 702-701 Korea 2Bio Industry Center Daegu Technopark Daegu 704-701 Korea 3Department of Food Science and Technology Keimyung University Daegu 704-701 Korea The effect of extracellular p- 1 3 1 6 -glucan produced by Paenibacillus polymyxa JB115 on nitric oxide NO production in macrophages was investigated. p -glucan induced the production of NO by macrophages in a concentration- and time-dependent manner. Moreover p-glucan stimulation increased the mRNA expression of iNOS COX-2 and IL-6 in macrophages in a concentration-dependent manner. Keywords p-glucan macrophages nitric oxide Paenibacillus polymyxa Introduction NO is induced during macrophage activation and thereby contributes to controlling the replication or neutralizing intracellular microbial pathogens 13 . Various studies indicated that NO is an important messenger in diverse biological functions including neuronal transmission vascular relaxation immune modulation and cytotoxicity against tumor cells 13 14 . p-glucans are heterogeneous groups of glucose polymers usually found in the cell walls of fungi 17 plants 11 and some bacteria 7 . They consist of linear p-1 3-linked D-glucose molecules with P-1 6-linked side chains of varying length occurring at different intervals along the backbone and can form complex tertiary structures stabilized by inter-chain hydrogen bonds 2 3 . Some animal studies addressed the beneficial effects of p-glucans on the growth performance of pigs 5 19 on the survival rate of mice challenged with Staphylococcus aureus or Candida albicans 16 and on the .
