Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Immunohistochemical localization of galectin-3 in the granulomatous lesions of paratuberculosis-infected bovine intestine | J. Vet. Sci. 2009 10 3 177-180 DOI JOURNAL OF Veterinary Science Immunohistochemical localization of galectin-3 in the granulomatous lesions of paratuberculosis-infected bovine intestine 1 1 2 1 1 1 3 1 Juyeon Lee Changjong Moon Jihoon Kim Chanwoo Jung Keun-Hwa Lee Hong-Gu Joo Meejung Ahn1 Taekyun Shin1 Department of Veterinary Anatomy College of Veterinary Medicine and Applied Radiological Science Research Institute Jeju National University Jeju 690-756 Korea 2Department of Veterinary Anatomy College of Veterinary Medicine and Animal Medical Institute Chonnam National University Gwangju 500-757 Korea 3Department of Microbiology College of Medicine Jeju National University Jeju 690-756 Korea The presence of galectin-3 was immunohistochemically quantified in bovine intestines infected with paratuberculosis Johne s disease to determine whether galectin-3 was involved in the formation of granulation tissue associated with the disease. Mycobacterium avium subsp. paratuberculosis infection was histochemically confirmed using Ziehl-Neelsen staining and molecularly diagnosed through rpoB DNA sequencing. Galectin-3 was detected in the majority of inflammatory cells possibly macrophages in the granulomatous lesions within affected tissues including the ileum. These findings suggest that galectin-3 is associated with the formation of chronic granulation tissues in bovine paratuberculosis probably through cell adhesion and anti-apoptosis mechanisms. Keywords bovine paratuberculosis galectin-3 granulation intestine Introduction Galectin-3 is a P-galactoside-binding animal lectin that contains carbohydrate-recognition domains and displays multiple related functions 2 . Extracellular galectin-3 mediates inflammation 1 whereas intracellular galectin-3 regulates cell growth and anti-apoptosis and modulates cell adhesion thus inducing cell migration 16 . Galectin-3 has been found in the cytoplasm of various cell types including inflammatory cells such as .
