Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Role of protease inhibitors and acylation stimulating protein in the adipogenesis in 3T3-L1 cells | J. Vet. Sci. 2009 10 3 197-201 DOI JOURNAL OF Veterinary Science Role of protease inhibitors and acylation stimulating protein in the adipogenesis in 3T3-L1 cells Mohamed Mohamed Soliman1 A Yakut Abdel-Fattah El-Senosi1 Maysara Mahmoud Salem2 Omniya Mahmoud Abdel Hamid1 Kimura Kazuhiro3 Department of Biochemistry Faculty of Veterinary Medicine Benha University 020-013 Egypt 2Department of Histology Faculty of Medicine Benha University 020-013 Egypt 3Department of Biomedical Sciences Laboratory of Biochemistry Graduate School of Veterinary Medicine Hokkaido University Sapporo 060-0818 Japan Treatment of AIDS HIV and hepatitis C virus needs protease inhibitors PI to prevent viral replication. Uses of PI in therapy are usually associated with a decrease in body weight and dyslipidemia. Acylation stimulating protein ASP is a protein synthesized in adipocytes to increase triglycerides biosynthesis for that the relation of PI and ASP to adipogenesis is tested in this work. ASP expression was increased during 3T3-L1 differentiation and reached a peak at day 8 with cell maturation. Addition of PI during adipocytes differentiation dose dependently and significantly p inhibited the degree of triglycerides TG accumulation. Moreover presence of ASP 450 ng mL in media significantly p stimulated the degree of TG accumulation and there was additive stimulation for ASP when added with insulin 10 1 g mL . Finally when ASP in different doses Low Medium 45 and High 450 ng mL incubated with a dose of 150 PI ASP partially inhibited the PI-inhibited adipogenesis and TG accumulation. The results in this study show that PI inhibit lipids accumulation and confirm role of ASP in TG biosynthesis and adipogenesis. Keywords acylation stimulating protein adipogenesis 3T3L-1 cells Introduction Protease inhibitors PI are a class of medications used to treat viral infections such as HIV AIDS and hepatitis C virus 2 . PI prevents viral replication by .
