Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Efficacy of strain RB51 vaccine in protecting infection and vertical transmission against Brucella abortus in Sprague-Dawley rats | J. Vet. Sci. 2009 10 3 211-218 DOI JOURNAL OF Veterinary Science Efficacy of strain RB51 vaccine in protecting infection and vertical transmission against Brucella abortus in Sprague-Dawley rats Md. Ariful Islam1 Mst. Minara Khatun1 Byeong-Kirl Baek1 Sung-Il Lee2 Korean Zoonoses Research Institute Chonbuk National Universily Jeonju 561-756 Korea 2Division of Model Animal Institute of Biomedical Science Kansai Medical University Osaka 570-8506 Japan Immunizing animals in the wild against Brucella B. abortus is essential to control bovine brucellosis because cattle can get the disease through close contact with infected wildlife. The aim of this experiment was to evaluate the effectiveness of the B. abortus strain RB51 vaccine in protecting infection as well as vertical transmission in Sprague-Dawley SD rats against B. abortus biotype 1. Virgin female SD rats n 48 two months of age were divided into two groups one group n 24 received RB51 vaccine intraperitoneally with 3 X 1010 colony forming units CFU and the other group n 24 was used as non-vaccinated control. Non-vaccinated and RB51-vaccinated rats were challenged with X 109 CFU of virulent B. abortus biotype 1 six weeks after vaccination. Three weeks after challenge all rats were bred. Verification of RB51-vaccine induced protection in SD rats was determined by bacteriological serological and molecular screening of maternal and fetal tissues at necropsy. The RB51 vaccine elicited protection in SD rats against infection with B. abortus biotype 1. Offspring from rats vaccinated with RB51 had a decreased p prevalence of vertical transmission of B. abortus biotype 1 compared to the offspring from non-vaccinated rats and respectively . This is the first report of RB51 vaccination efficacy against the vertical transmission of B. abortus in the SD rat model. Keywords Bruce-ladder multiplex PCR assay brucellosis stillbirth strain RB51 vaccine wildlife Introduction .
