Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp Original article đề tài:"How long can seeds of Norway spruce (Picea abies (L.) Karst.) be stored?" | Ann. For. Sci. 62 2005 73-78 INRA EDP Sciences 2005 DOI forest 2004082 73 Original article How long can seeds of Norway spruce Picea abies L. Karst. be stored Boleslaw SUSZKAa Pawel CHMIELARZa Reinhard WALKENHORSTb a Polish Academy of Sciences Institute of Dendrology 62-035 Kórnik Poland b JahnstraBe 11 63517 Rodenbach Germany Received 16 July 2003 accepted 31 March 2004 Abstract - Seeds of Norway spruce Picea abies L. Karst. belong to the orthodox category because they can be desiccated to a low level of moisture content and be stored at low temperature without damage. Here we present original results of germination tests and observations of seedling development after storage of seeds for the first 17 years at -5 C to -6 C followed by 12 years at -3 C in total for 29 years at subfreezing temperatures. We also investigated cryopreservation in liquid nitrogen at -196 C as an alternative method of seed storage. Before cryopreservation seeds were desiccated to a moisture content of 3 or 6 . After 2-year storage in liquid nitrogen their germination course and development of seedlings did not differ from those of freshly extracted and non-stored seeds and also from those of seeds stored for 2 years at -3 C. Picea abies conservation cryopreservation desiccation germination seedling growth Résumé - Combien de temps les graines d epicea Picea abies L. Karst. peuvent-elles être conservées Les graines d epicea appartiennent à la catégorie des graines dites orthodoxes parce qu elles peuvent être déshydratées jusqu a une faible teneur en eau et peuvent être conservées sans dommage à basse température. Nous présentons ici les résultats des tests de germination et des observations sur le développement des semis après stockage des graines pendant 29 ans de -5 à -6 C pendant 17 ans puis à -3 C pendant 12 ans. La cryoconservation dans l azote liquide -196 C une méthode alternative de stockage des graines a aussi été étudiée. Avant leur cryoconservation les graines ont été .
