Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí lâm nghiệp đề tài: Micrografting of mature stone pine (Pinus pinea L.) trees. | Ann. For. Sci. 61 2004 843-845 INRA EDP Sciences 2005 DOI forest 2004081 843 Note Micrografting of mature stone pine Pinus pinea L. trees Millán CORTIZOa Pablo ALONSOa Belén FERNÁNDEZa Ana RODRÍGUEZa Maria Luz CENTENOb Ricardo J. ORDÁSa a Unidad de Fisiología Vegetal Departamento de Biología de Organismos y Sistemas Universidad de Oviedo C Catedrático Rodrigo Uría s n 33071 Oviedo Spain b Departamento de Biologia Vegetal Universidad de León Spain Received 1 December 2003 accepted 31 August 2004 Abstract - This paper describes an in vitro micrografting method for selected mature Pinus pinea L. trees. Needle fascicles of five selected clones were micrografted onto hypocotyls of two weeks-old germinated isolated embryos. Fascicle meristems outgrowth was recorded after one month of culture and the performance of the clones assayed was evaluated. Clones behave statistically different in establishment and development rates. Overall success of our protocol reached 43 of the graftings made. micrografting micropropagation conifer in vitro grafting Pinus pinea Résumé - Micro-greffage de clones sélectionnés de Pinus pinea L. Ce rapport décrit une méthode pour le greffage in vitro de Pinus pinea L. Des brachyblastes de cinq clones ont été greffés sur embryons isolés germés âgés de deux semaines. Le développement des méristèmes fasciculaires a été quantifié pour chaque clone après un mois. Les pourcentages d établissement et de développement sont statistiquement différents pour les cinq clones. Le taux de succès du greffage réalisé par notre méthodologie s élève à 43 . micro-greffage micro-propagation conifer in vitro greffage Pinus pinea Stone pine Pinus pinea L. is one of the most important tree species of the Mediterranean area. It has gained new attention in the last years due to the rural development programs granted by the European Union. Highly regarded owing to its ecological and landscape value it lives in zones with bad soils and a strong summer drought. Stone
