Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp quốc tế đề tài: Effect of inoculation and substrate disinfection method on rooting and ectomycorrhiza formation of Douglas fir cuttings. | 35 Ann. For. Sci. 56 1999 35- M Inra Elsevier Paris Original article Effect of inoculation and substrate disinfection method on rooting and ectomycorrhiza formation of Douglas fir cuttings Javier Parladéa Joan Peraa Isabel F. Alvarez3 Daniel Bouchardb Benoit Généréc Franẹois Le Taconb Departament de Patologia Vegetal Ctra de Cabrils s n IRTA 08348 Cabrils Barcelona Spain bCentre de recherches íồrestièrès de Nancy Inra 54280 Champenoux France cCemagref Domaine des Barres 45290 Nogent-sur-Vernisson France Received 2 February 1998 accepted 1 1 May 1998 Abstract - Ectomycorrhizal inoculation techniques were applied to Douglas fir Pseudotsuga menziesii Mirb Franco cuttings during the rooting phase to determine the effect of inoculation on rooting and mycorrhizal colonization. Two experiments were conducted 1 an inoculation experiment established at three different locations with the same treatments to determine the effect of inoculation with Laccaria bicolor Melanogaster ambiguus and subareolatus and 2 a local factorial experiment to determine the effect of fungal inoculation substrate disinfection methods and their possible interactions. At Peyrat-le-Château and Champenoux France there were no differences in rooting due to the inoculation treatment. At Cabrils Spain the cuttings inoculated with L. bicolor and R subareolatus rooted significantly better than non-inoculated cuttings. Vegetative inoculum of L. bicolor and spores of R. subareolatus were effective in forming ectomycorrhizas in the three locations whereas spores of M. ambiguus were effective only at Cabrils Substrate disinfection with methyl bromide significantly decreased the percentage of rooted cuttings compared to steam-disinfected substrate. Methyl bromide application also lowered the percentage of mycorrhizal cuttings after inoculation with L. bicolor and R. subareolatits. The disinfection method did not alter significantly the colonization level among the mycorrhizal plants. Inra Elsevier .
