Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp quốc tế đề tài: Lack of mother tree alleles in zymograms of Cupressus dupreziana A. Camus embryos. | 17 Ann. For. Sci. 57 2000 17-22 INRA EDP Sciences 2000 Original article Lack of mother tree alleles in zymograms of Cupressus dupreziana A. Camus embryos Christian Pichot Bruno Fady and Isabelle Hochu INRA Unité de Recherches Forestières Méditerranéennes Équipe de Génétique Av. Vivaldi 84000 Avignon France Received 12 May 1999 accepted 13 September 1999 Abstract - Unexpected ploidy levels observed in a previous flow cytometric analysis of Cupressus dupreziana endosperm led us to hypothesize an asexual seed production in this endangered species. We tested here this possible apomixis studying isozyme variability of endosperms embryos and seed coats extracted from open pollinated seeds. Electrophoretic bands observed in zymograms of PGI2 and LAP the only two polymorphic systems were in agreement with bands observed in C. sempervirens zymograms. Zymograms of C. dupreziana endosperms were identical to those of the diploid maternal tissue as observed in C. sempervirens. Hypothesizing a codominant nuclear DNA control for both PGI2 and LAP a similar electrophoretic expression of these systems in endosperm and embryo and the absence of modifier genes the lack of endosperm bands in most embryo zymograms was interpreted as the result of a strictly paternal origin of embryo nuclear DNA in C. dupreziana seeds. Cupressus dupreziana isozyme reproduction gymnosperm paternal inheritance Résumé - Absence d alleles maternels dans les zymogrammes des embryons de Cupressus dupreziana A. Camus. De précé-dents travaux portant sur les niveaux de ploidie de l endosperme des graines de Cupressus dupreziana nous avaient conduit à émettre l hypothese d une reproduction apomictique chez cette espèce menacée. Cette hypothèse a été ici testée par l étude de la variabilité enzymatique d endospermes d embryons et de téguments de graines issues de pollinisation libre. Les zymogrammes observés pour les deux systèmes polymorphes PGI2 et LAP sont conformes à ceux observés chez C. sempervirens. Les .
