Tuyển tập các báo cáo nghiên cứu khoa học ngành y học tạp chí Medical Sciences dành cho các bạn sinh viên ngành y tham khảo đề tài: Laboratory diagnosis of Toxoplasma gondii infection. | Int. J. Med. Sci. 2009 6 135 International Journal of Medical Sciences 2009 6 3 135-136 Ivyspring International Publisher. All rights reserved Short Communication Laboratory diagnosis of Toxoplasma gondii infection A. Calderaro S. Peruzzi G. Piccolo C. Gorrini S. Montecchini S. Rossi C. Chezzi G. Dettori Department of Pathology and Laboratory Medicine Section of Microbiology University of Parma Parma Italy Published Toxoplasma gondii is an obligate intracellular protozoan responsible for infections throughout the world in a wide range of hosts including humans. Primary infection is usually subclinical but in some patients cervical lymphadenopathy or ocular disease can be present 1 . Infection acquired during pregnancy may cause severe damage to the fetus. In immunocompromised patients reactivation of latent infection can cause life-threatening encephalitis 1 . T. gondii infection can be diagnosed indirectly with serological methods and directly by Polymerase Chain Reaction PCR hybridisation isolation and histology. Whereas indirect serological methods are widely used in immunocompetent patients definitive diagnosis in immunocompromised people is mostly undertaken by direct detection of the parasite 1 . In our laboratory the diagnosis of infection by T. gondii is carried out by the detection of specific anti-Toxoplasma immunoglobulin IgM and IgG and to discriminate chronic from reactivated infection IgG avidity is also determined with VIDAS instrument bioMerieux France 2 moreover the diagnosis of toxoplasmosis using bioptic tissue samples blood and urine is done detecting T. gondii DNA by a Real-Time PCR Fluorescence Resonance Energy Transfer FRET targeting T. gondii 529 bp repeated region 3 . The extraction of DNA is performed from samples by the MagNA Pure LC instrument Roche Diagnostics Mannheim Germany according to the manufacturer s specifications. For FRET PCR we used the LightCycler Fast-StartDNA MasterPLUS Hybridization Probes Kit Roche .
