Tuyển tập các báo cáo nghiên cứu khoa học ngành y học tạp chí Medical Sciences dành cho các bạn sinh viên ngành y tham khảo đề tài: Gene expression analysis of human red blood cells. | Int. J. Med. Sci. 2009 6 156 International Journal of Medical Sciences 2009 6 4 156-159 Ivyspring International Publisher. All rights reserved Research Paper Gene expression analysis of human red blood cells Sveta Kabanova1 Petra Kleinbongard1 Jens Volkmer1 Birgit Andrée2 Malte Kelm1 Thomas W. Jax1 3 4 H 1. Department of Medicine Division of Cardiology and Angiology Universitatsklinikum Dusseldorf Heinrich-Heine-University 40225 Dusseldorf Germany 2. Biologisch-Medizinisches Forschungszentrum Heinrich-Heine-University 40225 Dusseldorf Germany 3. Profil Institut fur Stoffwechselforschung Hellersbergstrasse 9 41461 Neuss Germany 4. Klinik fur Kardiologie Herzzentrum Wuppertal Universitat Witten Herdecke Wuppertal Germany Both authors equally contributed in this work H Correspondence to Thomas W. Jax MD PhD Profil Institut fur Stoffwechselforschung Hellersbergstrasse 9 41461 Neuss Germany. . Tel 49 - 2131 - 4018 - 0 Fax. 49 - 2131 - 4018 - 577 Received Accepted Published Abstract Understanding of molecular mechanisms governing the enucleating phenomena of human erythrocytes is of major importance in both fundamental and applied studies. Total RNA n 7 from human RBCs purity of erythrocyte preparation 99 99 was tested using 2100 Bioanalyzer Agilent USA and transcribed to cDNA. Microarray analysis was performed with the Human Genome Focus GeneChip Affymetrix USA containing 8500 transcripts corresponding to 8400 human genes. Here we report that human RBCs contain typical eukaryotic RNA with 28S- and18S-rRNA standard bands. Microarray studies revealed the presence of transcripts of 1019 different genes in erythrocytic RNA. Gene Ontology analysis recognized 859 genes involved in general biological processes 529 genes for cellular metabolism 228 genes for signal transduction 104 genes for development 107 genes for immune response 62 genes for protein localization 53 genes for programmed cell death and 5 genes for
