Tuyển tập các báo cáo nghiên cứu khoa học ngành y học tạp chí Medical Sciences dành cho các bạn sinh viên ngành y tham khảo đề tài: HLA-DR regulation and the influence of GM-CSF on transcription, surface expression and shedding. | Int. J. Med. Sci. 2004 1 3 126-136 126 Research paper Received Accepted Published Abstract Key words Author biography Corresponding address International Journal of Medical Sciences ISSN 1449-1907 2004 1 3 126-136 2004 Ivyspring International Publisher. All rights reserved HLA-DR regulation and the influence of GM-CSF on transcription surface expression and shedding Sara E Perry1 Sobhy M Mostafa2 Richard Wenstone2 Alan Shenkin3 Paul J McLaughlin1 1. Department of Immunology University of Liverpool Liverpool UK. 2. Intensive Care Unit Royal Liverpool University Hospital Liverpool UK. 3. Department of Clinical Chemistry University of Liverpool Liverpool UK. Low surface HLA-DR expression is a feature in sepsis. However the mechanisms that regulate HLA-DR expression have not been elucidated. The current study investigates regulation of HLA-DR gene transcription post transcriptional events and shedding of surface HLA-DR as well as the regulation of HLA-DR by GM-CSF and an immunomodulatory cytokine. Plasma and PBMC were collected from septic patients and healthy volunteers. An ELISA was developed to measure soluble HLA. PCR techniques were used to determine HLA-DR mRNA levels and flow cytometry and fluorescent microscopy were used for measurement of surface expressed and intracellular HLA-DR. Septic patients fulfilling the criteria of the American College of Chest Physicians ACCP for sepsis were recruited for the study n 70 . HLA-DR was measured on three consecutive days days seven and fourteen. Patients were excluded from the study if on immunosuppressive therapy. Results Higher levels of shed HLA-DR were found in the plasma of septic patients compared to healthy controls. The level of HLA-DR mRNA was significantly lower in septic patients compared to healthy controls however an increased intracellular HLA-DR expression was observed. When HL-60 cells were treated with GM-CSF gene transcription surface expression and shedding
