Tuyển tập các báo cáo nghiên cứu khoa học ngành y học tạp chí Medical Sciences dành cho các bạn sinh viên ngành y tham khảo đề tài: Anti-tumorigenic and Pro-apoptotic effects of CKBM on gastric cancer growth in n | Int. J. Med. Sci. 2004 1 3 137-145 137 International Journal of Medical Sciences Research paper ISSN 1449-1907 2004 1 3 137-145 2004 Ivyspring International Publisher. All rights reserved Anti-tumorigenic and Pro-apoptotic effects of CKBM on Received Accepted Published gastric cancer growth in nude mice Vivian Yvonne Shin1 Wallace Hau-Leung So1 Edgar Shiu-Lam Liu2 Ying-Jye Wu2 Shiu-Fun Pang2 and Chi-Hin Cho1 1 Department of Pharmacology Faculty of Medicine The University of Hong Kong Hong Kong HKSAR China 2 CK Life Sciences Limited Hong Kong HKSAR China Abstract Natural botanical products can be integrated with western medicine to optimize the treatment outcome increase immune function and minimize the side effects from western drug treatment. CKBM is a combination of herbs and yeasts formulated based on traditional Chinese medicinal principles. Previous study has demonstrated that CKBM is capable of improving immune responsiveness through the induction of cytokine mediators such as TNF-a and IL-6. In this study we aimed to investigate the effect of this immunomodulatory drug on gastric cancer growth using a human xenograft model. Gastric cancer tissues were implanted subcutaneously into athymic nude mice followed by a 14-day or 28-day of CKBM treatment. Results showed that higher doses of CKBM or ml mouse day produced a dose-dependent inhibitory effect on gastric tumor growth after 28-day drug treatment. This was associated with a decrease of cellular proliferation by 30 with concomitant increase in apoptosis by 97 in gastric tumor cells when compared with the control group. In contrast CKBM showed no effect on angiogenesis in gastric tumors. This study demonstrates the anti-tumorigenic action of CKBM on gastric cancer probably via inhibition of cell proliferation and induction of apoptosis and provides future potential targets of this drug candidate on cancer therapy. Key words PCNA apoptosis angiogenesis .
