Tuyển tập các báo cáo nghiên cứu khoa học ngành y học tạp chí Medical Sciences dành cho các bạn sinh viên ngành y tham khảo đề tài: Comparison of osteogenic potentials of human rat BMP4 and BMP6 gene therapy using [E1-] and [E1-,E2b-] adenoviral vectors. | Int. J. Med. Sci. 2006 3 97 Research paper International Journal of Medical Sciences ISSN 1449-1907 2006 3 3 97-105 2006 Ivyspring International Publisher. All rights reserved Comparison of osteogenic potentials of human rat BMP4 and BMP6 gene therapy using E1- and E1- E2b- adenoviral vectors Hongwei Li 1 Jin Zhong Li 1 Debra D. Pittman 2 Andy Amalfitano 3 Gerald R. Hankins1 and Gregory A. Helm1 4 1 Departments of Neurological Surgery University of Virginia Health System Charlottesville Virginia 22908 USA 2 Genetics Institute Andover Massachusetts 01810 USA 3 Departments of Pediatrics and Human Genetics Duke University Medical Center Durham North Carolina 27710 USA 4 Departments of Biomedical Engineering University of Virginia Health System Charlottesville Virginia 22908 USA Corresponding address Jin Zhong Li . . Department of Neurological Surgery University of Virginia Health System P. O. Box 800212 Charlottesville Virginia 22908 USA Received Accepted Published Osteogenic potentials of some recombinant human bone morphogenetic protein BMP first-generation adenoviral vectors ADhBMPs are significantly limited in immunocompetent animals. It is Unclear what role expression of viral proteins and foreign proteins transduced by adenoViral vectors play in the host immune response and in ectopic bone formation. In this study two sets of experiments were designed and performed. First rat BMP6 cDNA were amplified sequenced and recombined in first-generation adenoviral vector ADrBMP6 . A comparison of human and rat BMP6 adenoviral vectors demonstrated identical osteogenic activities in both immunodeficient and immunocompetent rats. Second the activities of recombinant human BMP6 in E1- AEhBMP6 and E1- E2b- E1- E2b- AEGFP hBMP6 and E1- E2b- AEhBMP6 adenoviral vectors were compared in both in vitro and in vivo models. Similar activities of these two generations of BMP adenoviral vectors were found in all models. These .
